Journal: medRxiv
Article Title: In-vitro characterization of 2019-24 Influenza B Viruses reveals increased temperature-dependent fitness in later timepoints independent of antigenic drift
doi: 10.1101/2025.10.27.25338757
Figure Lengend Snippet: Low MOI Growth curves on human nasal epithelial cell (hNEC) cultures (MOI = 0.01) at 33°C (A) or 37°C (D) reveal significantly different kinetics in early and late time points. (B-C) Virus production at 33°C between (B) 2-48 hours post infection(hpi) or (C) 48-120 hpi. Virus production between at 37°C at (E) 2-48 hours post infection (hpi) or (F) 48-120 hpi. (G-J) Growth curves plotted by virus at 33°C and 37°C per panel. Statistical testing was performed by one way (AUC) or two-way (growth curves) ANOVA with Tukey post host test. Each graph combines two or three experiments with four hNEC wells per experiment.
Article Snippet: Human nasal epithelial cells (hNEC) (PromoCell) were cultivated as previously described.
Techniques: Virus, Infection